RESUMO
The beetles of the subtribe Oedionychina (Chrysomelidae, Alticinae) are the only ones that have the atypical giant and achiasmatic sex chromosomes, which are substantially larger than the autosomes. Previous cytogenetic analyses suggest a large accumulation of repetitive DNA in the sex chromosomes. In this study, we examined the similarity of X and Y chromosomes in four Omophoita species and compared genomic differentiation to better understand the evolutionary process and the giant sex chromosomes origin. Intraspecific genomic comparation using male and female genomes of O. octoguttata and interespecific analyses using genomic DNA of O. octoguttata, O. sexnotata, O. magniguttis, and O. personata were performed. In addition, whole chromosome painting (WCP) experiments were performed with X and Y chromosome probes of O. octogutatta. CGH analysis revealed great genomic similarity between the sexes and a sex-specific region on the Y chromosome, and interspecific analysis revealed a genomic divergence between species. In contrast, WCP results revealed that the sex chromosomes of O. octoguttata have high intra- and interspecific similarity with the studied species. Our data support a common origin under the canonical evolution of the sex chromosomes in this group, as they have high genomic similarity between them.
RESUMO
The aim of this study was to analyze the correlation between triglyceride (TG) serum levels in obese and non-obese patients in a simulated postprandial state. Both groups showed TG levels < 150 mg/dL when fasting. After 12 h fasting, the subjects ingested a lipid overload diet and blood samples were collected. The variation between fasting and the postprandial TG peak levels were analyzed. The peak of postprandial TG levels occurred 4 h after the lipid overload in both groups. When the subjects were not fasting, the majority of non-obese subjects remained within the range of normal TG values, but the values for the obese group remained elevated. There was a significant correlation between Body Mass Index (BMI) and TG at each time point until 2 h after the meal, but the data did not show a correlation after 3 h. According to the receiver-operating characteristics (ROC) curve, postprandial TG values were not a good predictor of obesity (based on BMI), but they were a predictor of non-obesity. This study reinforces the importance of measuring non-fasting TG levels in obese and non-obese subjects, because some non-obese patients probably had altered fat metabolism, indicating that this examination could be an indicator of metabolic risk.
